Butters
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| Detailed Information for Phage Butters | |
| Discovery Information | |
| Found by | Lena Ma |
| Found in | Philadelphia, PA USA |
| Finding Institution | Lehigh University |
| Year Found | 2011 |
| From enriched soil sample? | Yes |
| Program | SEA Program |
| GPS Coordinates | 40.06067 N, 75.046608 W Map |
| Discovery Notes | Phage was discovered from a soil sample in Philadelphia at 25 degrees Celcius. The temperature is sunny and 78% humidity. The soil sample collected was dry and obtained from soil 3cm deep. The soil was also near growing plants where there was only sunlight (no shade). |
| Plaque Notes | After 24 hours of incubation, the plaque sizes are all 0.5mm in diameter and turbid. After 48 hours of incubation the plaques grew to be two different sizes. The first size is 0.5mm-1.0mm in diameter and turbid. The second size is 2.0mm-3.0mm in diameter and turbid. Even with the two different sizes the plaques yield the same phage. After incubation for longer than 48 hours, the plaques are all 3.0mm in diameter and bullseye. The bullseye effect is only shown after incubation of longer than 48 hours. Titers were performed from one of the 0.5mm-1.0mm plaques and from one of the 2.0mm-3.0mm plaques and both titers yielded the same morphology as discussed above, thus concluding that even with the two sized plaques, the phage is the same.
The phage is a siphoviridae with an icosahedral head but looks almost spherical on the EM picture. It has a 3:1 tail:head ratio and has horizontal banding on its tail. There is also a small ball on the end of the tail. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 29, 2012 |
| Sequencing Facility | VCU |
| Shotgun Sequencing Method | 454 Only |
| Genome length (bp) | 41491 |
| GC Content | 65.8% |
| Character of genome ends | Defined physical ends |
| 3' Overhang Length | 13 bp |
| 3' Overhang Sequence | CCCGCCGCCCTCG |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | N |
| Other Cluster Members | |
| Subcluster | N/A |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Has been Annotated? | No |
| Is live and searchable in GenBank? | Not yet |
| Available Multimedia | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Archiving Info | |
| SEA Designator | 2011LEHIbuttersLM |
