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MrGordo

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Detailed Information for Phage MrGordo
Discovery Information
Found by Sean Kearney
Found in West Lafayette, IN USA
Finding Institution Purdue University
Year Found 2010
From enriched soil sample? Yes
Program SEA Program
GPS Coordinates 40.422000 N, 86.916056 W Map
Discovery Notes MrGordo was found about an inch below moist soil in a flower bed. The flower bed was located about ten feet in front of the east entrance of the Agricultural and Biological Engineering building on Purdue's Campus. The temperature on the day of collection was 12.2 C and it was overcast.
Plaque Notes Large plaques, approximately 5 mm in diameter. Circular, cloudy edges, clear center - outer ring formation. The outer rings developed more completely about 24 hours after the formation of the plaques. There was a large degree of variability in the plaque sizes (between 2 and 6 mm) and most of the plaques would form with bias towards one side of the plate. The plaques did keep a consistent morphology after the final purification round, but there was still variation in the plaque sizes.
Sequencing Information
Sequencing Complete? Yes
Date Sequencing Completed Jan 13, 2011
Sequencing Facility VCU
Shotgun Sequencing Method 454 Only
Genome length (bp) 50988
GC Content 63.8%
Character of genome ends Defined physical ends
3' Overhang Length 10 bp
3' Overhang Sequence CGGATGGTAA
Fasta file available? Yes: Download fasta file
Characterization
Cluster A
Other Cluster Members

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Abrogate (A1)

Aeneas (A1)

Anglerfish (A1)

Bethlehem (A1)

BillKnuckles (A1)

Bob3 (A1)

BPBiebs31 (A1)

Bruns (A1)

BuckeyeNut (A1)

Bxb1 (A1)

ConceptII (A1)

DD5 (A1)

Doom (A1)

Dreamboat (A1)

Euphoria (A1)

Fascinus (A1)

FlowerChild (A1)

Fushigi (A1)

Jasper (A1)

JC27 (A1)

KatAttack (A1)

KBG (A1)

KSSJEB (A1)

Kugel (A1)

Lesedi (A1)

Lockley (A1)

Magnito (A1)

Manatee (A1)

Marcell (A1)

Minion (A1)

MPlant7149 (A1)

MrGordo (A1)

Museum (A1)

Nepal (A1)

Pari (A1)

PattyP (A1)

Perseus (A1)

RidgeCB (A1)

SkiPole (A1)

Solon (A1)

Spite (A1)

Switzer (A1)

Target (A1)

Trouble (A1)

U2 (A1)

Violet (A1)

Goose (A10)

Severus (A10)

Twister (A10)

Buddy (A2)

Che12 (A2)

D29 (A2)

Epoch (A2)

Goethe (A2)

GridersLegacy (A2)

KingSt (A2)

L5 (A2)

Lincaica11 (A2)

Nemo (A2)

Pukovnik (A2)

RedRock (A2)

Scorpio7 (A2)

Stumpy (A2)

Trixie (A2)

Turbido (A2)

Tyrone (A2)

Bxz2 (A3)

Dumpling (A3)

FloMo (A3)

HelDan (A3)

JHC117 (A3)

Jobu08 (A3)

Methuselah (A3)

Microwolf (A3)

Misomonster (A3)

Norbert (A3)

Rockstar (A3)

Vix (A3)

Wonder (A3)

Arturo (A4)

Backyardigan (A4)

Dhanush (A4)

Eagle (A4)

Flapjack (A4)

Flux (A4)

ICleared (A4)

LHTSCC (A4)

Maverick (A4)

MeeZee (A4)

Mundrea (A4)

Peaches (A4)

Sabertooth (A4)

Shaka (A4)

TiroTheta9 (A4)

TygerBlood (A4)

Wile (A4)

Wizard007 (A4)

Airmid (A5)

Benedict (A5)

Chadwick (A5)

Cuco (A5)

ElTiger69 (A5)

George (A5)

LittleCherry (A5)

Theia (A5)

Tiger (A5)

Blue7 (A6)

DaVinci (A6)

EricB (A6)

Gladiator (A6)

Hammer (A6)

Jeffabunny (A6)

HINdeR (A7)

Timshel (A7)

Astro (A8)

Saintus (A8)

Smeadley (A8)

Alma (A9)

PackMan (A9)

Afua (Unknown)

BaxterTD (Unknown)

Brooklyn (Unknown)

Butters (Unknown)

HerbSipe (Unknown)

LaChupacabra (Unknown)

Loser (Unknown)

Nakoi (Unknown)

Norris007 (Unknown)

Ousia (Unknown)

Oxente (Unknown)

Pbnep65 (Unknown)

PhrostyMug (Unknown)

Sabinator (Unknown)

SargentsShorty (Unknown)

Scooter (Unknown)

Skpmule (Unknown)

SmallSoldier (Unknown)

Spheric (Unknown)

Star (Unknown)

Uffda (Unknown)

Zeeba (Unknown)

Subcluster A1
Morphotype Siphoviridae
Has been Phamerated? Yes
Has been Annotated? Yes
Gene List

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Number of Genes 90
Number of tRNAs 0
Number of tmRNAs 0
Is live and searchable in GenBank? Yes: JN020140
Available Multimedia
Plaque Picture Download
EM Picture Download
Archiving Info
SEA Designator 2010PURDmrgordoSMK
SEA Lysate Titer 1.6e9
Date of SEA Lysate Titering Nov 9, 2010