Mutaforma13
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| Detailed Information for Phage Mutaforma13 | |
| Discovery Information | |
| Found by | Hannah Huntley |
| Found in | Raleigh, NC USA |
| Finding Institution | North Carolina State University |
| Year Found | 2010 |
| From enriched soil sample? | Yes |
| Program | SEA Program |
| GPS Coordinates | 35.782161 N, 78.666205 W Map |
| Discovery Notes | I collected my soil sample on the morning of August 24th, 2010. It was a cool morning, and it was lightly raining, therefore my soil sample was cool and moist. The air temperature was about 70 degrees.
The soil was under a thin layer of mulch, and I collected my sample from about an inch under the soil surface. |
| Plaque Notes | At high concentrations, my phage seems to exhibit characteristics
of a temperate life cycle. The plaques are small (1-2 mm in diameter) and turbid. At lower concentrations, however, my phage seems to have a lytic life cycle. The plaques are larger (3 mm in diameter) and are clear and round. They also have a bulls-eye ring around them. I think that the variation in plaque morphology may be due an ability of the phage to communicate with others of its kind through chemical signaling. Maybe its life cycle and replication rate are controlled by a protein that it secretes depending on the concentration of itself in its environment. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 22, 2011 |
| Sequencing Facility | DHMRI |
| Shotgun Sequencing Method | 454 Only |
| Genome length (bp) | 57701 |
| GC Content | 61.3% |
| Character of genome ends | Defined physical ends |
| 3' Overhang Length | 10 bp |
| 3' Overhang Sequence | CGGTAGGCGC |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | F |
| Other Cluster Members |
Click to ViewArdmore (F1) Bobi (F1) Boomer (F1) Braemar (F1) Cakepop (F1) Che8 (F1) Chikondi (F1) Clover (F1) CoolGuy (F1) Dante (F1) DeadP (F1) DLane (F1) Dorothy (F1) DotProduct (F1) Drago (F1) Fruitloop (F1) Gardregs (F1) Garlic (F1) Ginona (F1) GUmbie (F1) Hades (F1) Hamulus (F1) Hegedechwinu (F1) Ibhubesi (F1) Job42 (F1) Kalikimaka (F1) Krakatau (F1) Libby (F1) Llij (F1) Luigi (F1) Lysinator (F1) Mantra (F1) Mozy (F1) Mutaforma13 (F1) Ogopogo (F1) Pacc40 (F1) PhrankthaTank (F1) Plackberry (F1) PlayDoh (F1) PMC (F1) Prometheus (F1) Ramsey (F1) RockyHorror (F1) Saal (F1) SaltyMike (F1) SG4 (F1) Shauna1 (F1) ShiLan (F1) SiSi (F1) Spartacus (F1) Spoonbill (F1) Taino (F1) Taj (F1) Tweety (F1) Wee (F1) YoungOne (F1) Avani (F2) Che9d (F2) Yoshi (F2) Kishmeesh (Unknown) |
| Subcluster | F1 |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Has been Annotated? | Yes |
| Gene List |
Click to View |
| Number of Genes | 105 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Is live and searchable in GenBank? | Yes: JN020142 |
| Available Multimedia | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| DNAMaster File | Download |
| Archiving Info | |
| SEA Designator | 2010NCSUmutaforma13HDH |
| SEA Lysate Titer | 1.6e11 |
| Date of SEA Lysate Titering | Sep 28, 2010 |
