Preparation: Protocols and instructions to get set up to begin phagehunting. Includes information on how/where to order lab supplies, how to make media, safety precautions, naming guidelines, etc.
| Protocol Title | Last Modified | Description |
| Ordering Information for Common Supplies Download pdf |
Nov 14, 2011 | This protocol contains the vendors from which we order the most common supplies, as well as the catalog numbers of those items. |
| Preparing Phage Buffer Download pdf |
Nov 14, 2011 | Prepare standard buffer for storing and diluting mycobacteriophages. |
| Preparing MBTA Download pdf |
Nov 14, 2011 | Prepare MBTA stock for later use in plating bacterial lawns. |
| Preparing 7H9 Download pdf |
Nov 14, 2011 | Prepare the base broth for use in growing mycobacterial cultures and plating mycobacterial lawns. |
| Preparing ADC Download pdf |
Nov 14, 2011 | Prepare ADC for use as the energy source for mycobacterial growth in 7H9 cultures or 7H10 plates. |
| Preparing Working Media Download pdf |
Nov 14, 2011 | Finish preparing several common reagents (Phage Buffer, 7H9, MBTA) by adding final ingredients. |
| M. smegmatis Media Additions Download pdf |
Nov 14, 2011 | A reference table for the amount of additives to be added to media of M. smegmatis. |
| Preparing 0.2% Glucose Agar Plates Download pdf |
Nov 14, 2011 | Prepare 0.2% glucose agar plates for growing M. smegmatis. This is a more economical alternative that is now used in place of ADC plates. |
| Growing Mycobacterium smegmatis mc2 155 Download pdf |
Nov 14, 2011 | To grow and maintain a plate stock of M. smegmatis mc2 155, obtain a smooth starter culture for amplification using Tween®80, and grow bacteria for experiments. |